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Most sites do not allow bots.
How do poker sites detect bots?
I figured they scanned memory like how HUDs are detected.
Based on an answer on another question they also detect it on the server side.
It seems to me if you put some randomness in the play which you should do anyway it would be very hard to detect.
From what I know they track certain metrics to determine bots. Most bots are pretty damn weak but as individuals are often hard to detect. What they track tend to be the following:
In general bots are used in conjunction with other bots, and when they're used together it becomes easier to track. They track stats and trends in general. Like for example if at micro stakes, the average player will raise 6% of the time, now imagine you have several 'players' who are opening 25% of the time. It's likely not a coincidence.
Some poker sites have code built into their clients that allow them to display a captcha challenge when the server requests it (which likely is manually triggered by the security team after reports from other users, or automatically if your playing behavior shows suspicious metrics as detailed by @Grinch91). And some also do track mouse movement to see if it moves in a natural fashion like a human would.
I've never been shown one personally (I don't use a bot however :P) but I've seen screenshots and also some files/symbols with very informative names while skimming through the program files and the binary code of the executables.
Captchas aren't bulletproof though, and are likely only being used as another indication if a particular account might be operated by a bot. Services exist (in third-world countries, where labor is cheap) where you pay a cent fee to have a captcha solved for you by a real human. They provide APIs to integrate their service into automated workflows.
